[vc_row][vc_column css=”.vc_custom_1543924365737{padding-right: 25px !important;padding-left: 25px !important;}”][vc_column_text]Technology is the set of techniques, instruments and systems used to discover, describe and explain different phenomena. The pulmonary hypertension research group has developed and optimized several technologies to understand the disease and to discover treatments aimed at improving the quality of life of patients.<\/p>\n
We divide these different techniques into two categories: basic research and clinical research.[\/vc_column_text][vc_empty_space height=”30px”][vc_single_image image=”16245″ img_size=”full”][vc_empty_space height=”60px”][\/vc_column][\/vc_row][vc_row][vc_column][vc_tta_tabs style=”insignia_tab_layout3 title-font” active_section=”1″][vc_tta_section title=”Fundamental research” tab_id=”fundamental-research”][vc_empty_space height=”30px”][vc_row_inner equal_height=”yes” gap=”30″][vc_column_inner width=”1\/2″ css=”.vc_custom_1603289198290{margin-right: 50px !important;}”][vc_column_text]<\/p>\n
The laboratory is equipped with a high-performance inverted microscope (Axio Observer, Zeiss) with a module for 3D sectioning and an incubation chamber. It allows the analysis of several types of stains.[\/vc_column_text][vc_column_text]<\/p>\n
Immunofluorescence histology<\/strong><\/p>\n [\/vc_column_text][vc_single_image image=”16393″ img_size=”large” alignment=”center” onclick=”link_image”][vc_single_image image=”16391″ img_size=”large” alignment=”center” onclick=”link_image”][vc_column_text]<\/p>\n Histology by colorimetry<\/strong><\/p>\n [\/vc_column_text][vc_single_image image=”16387″ img_size=”large” alignment=”center” onclick=”link_image”][vc_single_image image=”16389″ img_size=”large” alignment=”center” onclick=”link_image”][vc_empty_space height=”120px”][vc_column_text]<\/p>\n The ChemiDoc is an imaging system for the detection of chemiluminescence. It is used for the visualization of proteins isolated from biopsies or cells in culture.[\/vc_column_text][vc_single_image image=”16385″ img_size=”large” alignment=”center” onclick=”link_image”][vc_empty_space height=”120px”][vc_column_text]<\/p>\n Real-time PCR is a technique that involves amplifying DNA fragments in order to quantify them.\u00a0This method, widely used in research, requires DNA from a few cells in culture or from a biopsy and provides important information for the identification of potential therapeutic targets.[\/vc_column_text][vc_empty_space height=”120px”][vc_column_text]<\/p>\n The Zeiss AxioScan.Z1 slide scanner enables automated scanning of up to 100 microscope slides (brightfield or fluorescence) into high quality virtual slides.[\/vc_column_text][vc_single_image image=”16084″ img_size=”large” alignment=”center” onclick=”link_image”][\/vc_column_inner][vc_column_inner width=”1\/2″ css=”.vc_custom_1603289206063{margin-left: 50px !important;}”][vc_column_text]<\/p>\n The use of human cells isolated from patients with pulmonary hypertension is essential for research.\u00a0The identification of potential therapeutic targets requires molecular and epigenetic analyzes of these cells.[\/vc_column_text][vc_single_image image=”16397″ img_size=”large” alignment=”center” onclick=”link_image”][vc_empty_space height=”120px”][vc_column_text]<\/p>\n This technology allows the measurement of oxygen consumption and extracellular acidification, two important indicators for cellular respiration and glycolysis.\u00a0Combined, this information tells us about the metabolic functions of cells.[\/vc_column_text][vc_single_image image=”16395″ img_size=”large” alignment=”center” onclick=”link_image”][vc_empty_space height=”120px”][vc_column_text]<\/p>\n The histology unit is a platform belonging to the pulmonary hypertension research group and available to the entire research center.\u00a0It includes all the devices necessary for specimen fixation, paraffin embedding and cutting for microscopic analysis.\u00a0For more information on the histology unit, to know the prices or to make a reservation, contact us at groupe.recherche.htap@criucpq.ulaval.ca<\/p>\n Available devices<\/p>\n [\/vc_column_text][vc_single_image image=”15750″ img_size=”large” alignment=”center” onclick=”link_image”][vc_empty_space height=”120px”][vc_column_text]<\/p>\n Tomography is an imaging technique using X-rays that allows us to learn a lot about the physiology of the disease.[\/vc_column_text][vc_single_image image=”16399″ img_size=”large” alignment=”center” onclick=”link_image”][vc_empty_space height=”120px”][vc_column_text]<\/p>\n Cardiac catheterization is an essential test for the diagnosis of pulmonary hypertension.\u00a0It consists of inserting a pressure and volume sensor into the pulmonary artery.\u00a0This procedure is used both clinically by the doctor for the diagnosis of the disease, and in basic research to verify the effectiveness of the treatments under study.[\/vc_column_text][\/vc_column_inner][\/vc_row_inner][\/vc_tta_section][vc_tta_section title=”Clinical research” tab_id=”clinical-research”][vc_empty_space height=”30px”][vc_row_inner equal_height=”yes” gap=”30″][vc_column_inner width=”1\/2″ css=”.vc_custom_1603289198290{margin-right: 50px !important;}”][vc_column_text]<\/p>\n Magnetic stimulation of the femoral nerve causes an involuntary contraction of the quadriceps.\u00a0The force of voluntary contraction of the quadriceps is compared to the force of involuntary contraction generated by this device, which is used as a maximum reference measurement.\u00a0The BiStim is directly connected to an amplifier and an analysis module.[\/vc_column_text][vc_empty_space height=”120px”][vc_column_text]<\/p>\n This robotic and computerized dynamometer makes it possible to measure muscle strength by applying different types of loads (isometric, isokinetic, isotonic).\u00a0In research, it is mainly used to assess the strength and endurance of the quadriceps, but its applications are wide (post-injury rehabilitation, identification of the risk of falling, neurorehabilitation, etc.)[\/vc_column_text][vc_empty_space height=”120px”][vc_column_text]<\/p>\n description to come<\/em>[\/vc_column_text][vc_empty_space height=”120px”][vc_column_text]<\/p>\n Combined with a respiratory analysis module (Vmax 29 spectra), the ergocycle is mainly used in the clinical laboratory to determine cardiopulmonary capacity.\u00a0However, it can be used for a multitude of exercise protocols such as a constant load endurance test or VO2 Max test.[\/vc_column_text][vc_empty_space height=”120px”][vc_column_text]<\/p>\n The portable nature of this device makes it possible to measure the gas exchanges of each breath while the subject is moving.\u00a0It can therefore monitor several physiological parameters (heart rate, VO2, VCO2, minute ventilation, saturation, etc.) continuously during outpatient tests.[\/vc_column_text][vc_empty_space height=”120px”][vc_column_text]<\/p>\n This system measures the variation in muscle and \/ or cerebral oxygenation and the variation in blood volume.\u00a0We can also get muscle oxygen saturation data and muscle oxygen consumption.[\/vc_column_text][\/vc_column_inner][vc_column_inner width=”1\/2″ css=”.vc_custom_1603289206063{margin-left: 50px !important;}”][vc_column_text]<\/p>\n Digital arterial pulse plethysmography allows constant measurement of arterial pressure and advanced hemodynamic variables.[\/vc_column_text][vc_empty_space height=”120px”][vc_column_text]<\/p>\n Placing the electromyograph electrode on a muscle measures electrical activity there.\u00a0This makes it possible to determine the muscles recruited, their degree of activation and the order in which they are recruited.[\/vc_column_text][vc_empty_space height=”120px”][vc_column_text]<\/p>\n The Vmax Encore with plethysmography module allows you to perform tests such as spirometry, diffusion capacity, measurement of lung volumes, lung compliance as well as inspiratory and expiratory pressure.\u00a0It is also possible to measure total lung capacity by removing nitrogen with the Platinum Body Plethysmograph.[\/vc_column_text][vc_empty_space height=”120px”][vc_column_text]<\/p>\n This surgical procedure is performed under local anesthesia by an experienced doctor.\u00a0A thin incision allows the insertion of a biopsy trocar which collects a muscle sample.\u00a0This is then analyzed in the fundamental laboratory.[\/vc_column_text][\/vc_column_inner][\/vc_row_inner][\/vc_tta_section][\/vc_tta_tabs][vc_empty_space height=”60px”][\/vc_column][\/vc_row]<\/p>\n","protected":false},"excerpt":{"rendered":" [vc_row][vc_column css=”.vc_custom_1543924365737{padding-right: 25px !important;padding-left: 25px !important;}”][vc_column_text]Technology is the set of techniques, instruments and systems used to discover, describe and explain different phenomena. The pulmonary hypertension research group has developed and […]<\/p>\n","protected":false},"author":1,"featured_media":0,"parent":16368,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_monsterinsights_skip_tracking":false,"_monsterinsights_sitenote_active":false,"_monsterinsights_sitenote_note":"","_monsterinsights_sitenote_category":0,"footnotes":""},"class_list":["post-16376","page","type-page","status-publish","hentry"],"yoast_head":"\nChemiDoc<\/h5>\n
qRT-PCR<\/h5>\n
Zeiss AxioScan.Z1 slide scanner<\/h5>\n
Cellular culture<\/strong><\/h5>\n
Seahorse XFe24 technology<\/h5>\n
Histology unit<\/h5>\n
\n
Tomography<\/h5>\n
Cardiac catheterization<\/h5>\n
BiStim magnetic stimulator<\/h5>\n
Biodex System 4 pro<\/h5>\n
Non-ferromagnetic ergocycle<\/h5>\n
Ergocycle with electromagnetic brake<\/h5>\n
Portable telemetry system for respiratory physiology assessment<\/h5>\n
Near-infrared spectroscopy (NIRS)<\/h5>\n
Non-invasive continuous heart monitoring<\/h5>\n
Electromyography<\/h5>\n
Complete analysis of respiratory functions<\/h5>\n
Muscle biopsy<\/h5>\n